Protein Drug Design and Molecular Diagnostics

The latest achievements of Dr. Yaoqi Zhou,Dr. Jian Zhan and their collaborators: high-throughput gene cutting to discover protein complementary fragments
Posted onFeb 08,2022

If a gene is cut into two segments and translated into two protein segments respectively, will it still have its original function? Experiments have found that most protein fragments will lose their functions, a few can automatically combine to maintain their original functions, and some require other auxiliary measures (such as distance constraints) to maintain their original functions. The former are called self-complementary fragments and the latter are called assisted or conditional complementary fragments. Auxiliary complementary fragments are important tools for probing biomolecular interactions. However, finding suitable cutting points and finding auxiliary complementary fragments with high signal-to-noise ratio is extremely time-consuming and labor-intensive, and the success rate is low. At present, only less than 20 auxiliary complementary variants have been found. Dr. Jian Zhan and Dr. Yaoqi Zhou of Shenzhen Bay Laboratory worked with Dr. Kai Zhou (first author), Dr. Thomas Litfin (co-first author), Solayman PhD student (co-first author) and Professor Huijun Zhao at Griffith University In cooperation, a high-throughput HiTS (High-Throughput Split-protein profiling) technology has been successfully developed, which can quickly discover self-complementary and auxiliary-complementary fragments. The study was published in International Journal of Biological Macromolecules 203, 543-552 (2022).For details, please click: link